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KMID : 0357819930170020001
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Korean Journal of Legal Medicine
1993 Volume.17 No. 2 p.1 ~ p.7
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Deoxyribonucleic Acid(DNA) Typing from Signle Hair
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Abstract
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Hairs are one of the biological evidences which are found frequently at crime scenes, so their identification can be of considerable forensic importance. DNA were extracted from shaft as well as root of a single hair for DNA typing. The root end
of
a
plucked single hair contains 120-200 ng DNA, whereas hair shaft contains too little DNA to determine its quantity. Using the polymerase chain reaction(PCR), genotypes of HLA DQA1 locus and AMP-FLPs(amplified fragment length polymorphisms)of D1S80
and
COL2A1 locus were determined from hair shaft and hair root.
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KEYWORD
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